402 Gut Microbiome Dysbiosis index: a novel approach to quantify the degree of gut dysbiosis in patients with chronic plaque psoriasis

Abstract

Gut microbiome was shown to have a significant impact in various inflammatory skin diseases including psoriasis through gut-skin axis. Microbiome index derived from gut microbiome taxonomic signatures were used predict the health status of gut in chronic diseases in previous studies. Ten microbiome signatures (Atopobium parvulum, Clostridium ramosum, Fusobacterium nucleatum, Gemella morbilloru, Granulicatella adiacens, Lachnospiraceae bacterium, Peptostreptococcus stomatis, Solobacterium moorei, Streptococcus gordonii, Subdoligranulum variabile) was chosen from previous studies and we developed a proprietary qPCR panel with a novel algorithm of gut microbiome dysbiosis index calculation, where the index (range: 0-100) was defined by the summation of the level of aberrant abundance in term of ΔCt (normal=0; moderate=1; severe=2) the fold change of prevalence between healthy and non-healthy population.