40-OR

Abstract

Genetic control of cytokine production is a feature of variation in cytokine gene regulatory regions that produce low, moderate, or high cytokine production profiles. We have earlier shown that renal allograft recipients carrying low Interleukin-10 (IL-10) producing genotypes have high incidence of chronic allograft failure and graft inflammation. In the present study of a 15-year post transplant follow up, we report the impact of IL-10 gene variants on renal allograft survival. A total of 218 renal transplant recipients with a maximum of 15-year follow-up were recruited. Three variants of IL-10 promoter region (-1082G/A, -819C/T, -592C/A) were analyzed by Luminex based sequence-specific oligonucleotide probe method. Kaplan-Meir (with Log-rank test) and Cox-regression survival analyses using confounding factors like HLA matching, acute rejection as independent variables were performed to assess the impact of IL-10 gene variants on graft survival. Renal transplant recipients carrying low IL-10 producing genotypes had significantly lower graft survival than recipients carrying high IL-10 producing genotypes (65% v/s 92%; p = 0.001; Hazard ratio = 4.35) [Fig. 1]. Renal transplant recipients carrying high IL-10 genotypes have significant better allograft survival. The likely explanation is high IL-10 production contributing to an efficient regulatory immune environment leading to controlled allo-immune responses and better allograft survival.