Abstract
On December 31, 2019, the Chinese health authorities notified the World Health Organization (WHO) of the appearance of cases of pneumonia of unknown etiology in the city of Wuhan, Hubei- China..
Highlights
4) A difference of 10 °C with respect to the annealing temperature of the first pair of RdRp primers when it should be 2 °C
8) The PCR test does not contain a single positive control to evaluate its specificity for SARS-CoV-2 or a negative control to exclude the presence of other coronaviruses, which makes the test unsuitable as a specific diagnostic tool to identify SARSCoV-2
Bruno, et al [7]. conducted another critical review of the article by Corman, et al [4] focused on the “Non-specificity of the Real Time RT-PCR test to detect COVID-19” in which to show the inability of the RT-PCR test to discriminate between different coronavirus strains and to confirm the molecular diagnosis of infection with the novel SARS-CoV-2 virus
Summary
4) A difference of 10 °C with respect to the annealing temperature of the first pair of RdRp primers (direct and reverse) when it should be 2 °C. This leads to an increase in nonspecific binding, amplification, and lack of specificity to identify the SARS-CoV-2 virus.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biomedical Journal of Scientific & Technical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
