Abstract
Publisher Summary This chapter focuses on the ubiquinol-cytochrome-c oxidoreductase from saccharomyces cerevisiae . As a consequence, such strains have to be identified among the broader class of respiratory-deficient nuclear petite (pet) mutants. The chapter discusses the screening for cytochrome-deficient mutants. Mutations are transferred to the strains of opposite mating type to that of the original pet isolates by crosses to a respiratory competent haploid with complementing auxotrophic markers. Following sporulation, the meiotic progeny are checked for the desired mating type, auxotrophy, and the presence of the pet mutation either by tetrad or random spore analysis. The chapter also describes the status of cytochrome b. All the nuclear genes for the constituent polypeptides of the yeast complex have been cloned and natural mutants or construct with null mutations in each gene are available.
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