4 Survey of Mycoplasma Wenyonii Infection in a Texas Beef Cattle Herd

Abstract

Abstract Mycoplasma wenyonii is hemotrophic bacteria known to cause mild anemia, localized edema, and pyrexia in infected cattle. It has been previously detected in cattle herds across the world, including United States beef herd. Mode of transmission, prevalence, and a full profile of symptoms associated with this species of bacteria have not been established. This case study aimed to elucidate the percentage of infected cattle in a local Erath County beef herd and determine which groups of cattle may have the greatest infection rates. Groups were determined based on sex and age, bull, cow, or unsexed calf. Blood was collected from 4 bulls, 61 cows, and 55 calves from the same producer on the same day. A blood extraction kit that utilized spin column-based nucleic acid purification was used to extract DNA from blood samples. Primers were constructed to be highly specific to the m. wenyonii sequence to avoid confusion with other common mycoplasma species. Extracted m. weyonii DNA was quantified using qPCR. Melt curve analysis was performed to verify the amplicon product size. Standard deviation of the melting point was calculated and selection was made within 2 standard deviations of the control. Animals with any detectable amount of m. wenyonii DNA present in their blood were considered positive for infection for the purposes of this case study. This survey yielded positive results for 2 of the 4 bulls, 19 of the 61 cows, and 0 calves. The lack of positive samples in the calf population suggests that the placenta functions as a barrier to infection. This study provides perspective on the prevalence of this microorganism in Texas cattle populations and serves as a basis for future studies. More investigation is needed to better understand the impact of this bacterial infection on Texas beef cattle producers.