Abstract
This chapter discusses the emerging picture of the DNA duplexes in solution as seen by the modern nuclear magnetic resonance (NMR) methods. Commonly available experimental NMR data alone are not sufficient to determine an accurate average structure of a DNA duplex in solution with high definition. A theoretical force field must be employed in the refinement process, together with experimental restraints, to produce solution structures in agreement with independent data. Importantly, the nature of a force field does not appear to be essential; using different empirical force fields but the same experimental restraints during the refinement has led to practically the same DNA conformations. The overall average conformation of DNA oligonucleotides in solution is B-form. At the same time, modern NMR methods are able to detect a strong sequence-dependent local heterogeneity of the solution DNA structure similar to the high-resolution X-ray methods for DNA in solid state.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.