Abstract
Publisher Summary This chapter discusses the synthesis, stability, storage, and some properties of photoreactive hormones. The applications of photoaffinity-derivatized hormones are described to highlight the potential uses of this method. Methodology related to gonadotropin releasing hormone (GnRH), insulin, and adrenocorticotropic hormone (ACTH) receptors are presented. The most widely used photolabel probes are the aryl azides. The advantage of photogenerated nitrene intermediates is that they are highly reactive and are capable of insertion into all protein amino acid side chains. Aryl azide derivatives are stable in the solid state or in solution. The excessive heating and strong oxidizing or reducing conditions should be avoided during experimental procedures. Photoaffinity labeling has evolved into a widely used technique for studying complex biological systems. These include photoaffinity inactivation of receptors; persistent activation of biological functions; identification of receptor macromolecules; and localization, processing, and regulation of receptors.
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