Abstract
Publisher Summary Periodate oxidations are used for structural analysis, particularly for the study of carbohydrates. Methods are described for estimation of the oxidation products, those most frequently encountered including formic acid, formaldehyde, and carbon dioxide. When carbon dioxide is formed during periodate oxidation of sugars and sugar acids, this product usually arises as a result of oxidation of α-carbonyl acid intermediates. Under conditions of strong acidity, periodic acid will not oxidize α-carbonyl acids. The steps described in the procedure include (1) periodate oxidation, (2) termination of periodate oxidation, (3) formation of p-nitrophenylhydrazones, (4) extraction of p-nitrophenylhydrazones from aqueous phase, (5) extraction of carbonyl acid p-nitrophenylhydrazones, (6) determination of concentration of extracted carbonyl acid, (7) reduction prior to periodate oxidation; and (8) determination of carbonyl acids prior to periodate oxidation. The use of p-nitrophenylhydrazine in place of 2,4-dinitrophenylhydrazine also makes it possible to compare directly results of carbonyl acid determinations with and without prior periodate oxidation. The orbital mixer used for extractions between aqueous and organic solvent phases has proved to be extremely efficient and eliminates the need for an aeration mixing apparatus.
Full Text
Topics from this Paper
Periodate Oxidation
Carbonyl Acid
Prior Periodate Oxidation
Organic Solvent Phases
Aqueous Phase
+ Show 5 more
Create a personalized feed of these topics
Get StartedSimilar Papers
Journal of Histochemistry & Cytochemistry
Dec 1, 1972
Analytical Biochemistry
Aug 1, 1962
Energy & Fuels
Feb 15, 2016
Macromolecules
May 19, 2004
Journal of Pharmaceutical Sciences
Nov 1, 2004
Fresenius Journal of Analytical Chemistry
Feb 1, 1986
Carcinogenesis
Jun 1, 1989
Electrochimica Acta
May 1, 2019
Biochimica et Biophysica Acta (BBA) - Biomembranes
Jul 1, 1972
International Journal of Pharmaceutics
Apr 1, 1988
Catalysis Today
Jun 1, 2015
Fluid Phase Equilibria
May 1, 1997
The Histochemical Journal
Sep 1, 1993
Separation and Purification Technology
Jun 1, 2020
Jan 1, 2004