Abstract
The Escherichia coli pdxC( serC) gene codes for a transaminase (EC 2.6.1.52). The gene is involved in both pyridoxine (vitamin B 6) and serine biosynthesis and was over-expressed as a MalE/ PdxC( SerC) fusion protein. The fusion protein was purified by affinity chromatography on an amylose resin and hydrolyzed in the presence of protease factor Xa. Both the fusion protein and the PdxC(SerC) protein were characterized ( K M value, turnover number, optimum pH). Both enzymes used 4- O-phosphoryl- l-threonine rather than 4-hydroxy- l-threonine as a substrate indicating that the phosphorylated rather than the non-phosphorylated amino acid is involved in pyridoxine biosynthesis. Pyridoxal phosphate was shown to be the cofactor for both enzymes and therefore seems to be involved in its own biosynthesis.
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