Abstract

Highly sensitive metallofluoreacent indicators are formed by condensing 4,4'-diaminostilbene and 4,4'-diaminostilbene-2,2'-disulphonic acid with sodium chloroacetate. The resulting N,N,N',N'- tetraacetic acids in the form of their sodium salts are used as indicators in the titration of copper with EDTA down to 0.001 M concentration. A number of metal ions can be determined by a back-titration procedure in which an excess of EDTA is titrated with coppcr(II) in the presence of the indicators. The end-point is marked by the appearance (or disappearance in back-titration) of the blue fluorescence.

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