Abstract
A new compound 4-[2-allylsulfanyl-1-(carboxymethyl-carbamoyl)-ethylcarbamoyl]-2-amino-butyric acid (ASAB) bearing allyl group was synthesized and characterized by spectroscopic (FTIR, NMR, & LC–MS) methods. ASAB showed significant antibacterial activity against gram-positive (S. aureus) and gram-negative (E. coli) bacteria. ASAB was screened for interaction with type I and II DNA topoisomerase activity via in vitro supercoil relaxation assay. ASAB was found to be a strong inhibitor for topoisomerases; with much more significant inhibition for topoisomerase II than topoisomerase I. Furthermore, DNA binding properties of ASAB with pBR322 DNA was investigated by fluorescent spectra which revealed its DNA binding ability. Molecular docking study was performed to elucidate the specific binding site in the active site of topoisomerase I & II.
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