Abstract
Department of Animal Science & Biotechnology, Gyeongnam Nationa l University of Science and Technology, Jinju 660-758, KoreaReceived September 11, 2014/Revised October 8, 2014/Accepted October 14, 2014The purpose of this study was to determine the effect of selenate on adipocyte differentiation and to identify genes involved in the modulation of adipogenesis in 3T3-L1 cells. To test the effect of selenate on adipocyte differentiation, adipogenesis was induced in cells using various concentrations (0–00 μM) of selenate. Various phases of adipogenesis were induced: postconfluent (PC), early phase (EP, d0-d2), postmitotic growth arrest (PM, d2-d4), and all period (AP). The PC cells exposed to selenate for 24 h displayed dose-dependent inhibition of intracellular lipid droplet accumulation on day 6 of adipogenesis. Two days of selenate treatment at EP or AP inhibited adipogenesis, with an approx-imately 20–80% reduction in lipid accumulation compared to that of a control (p<0.05). When pre-adipocytes were exposed to selenate during the PM period, the antiadipogenic effect of selenate was attenuated. Two types of selenoprotein genes (Seps1 and Sepp1) were up-regulated by the selenate treatment during mitotic clonal expansion, whereas these genes were down-regulated during PM growth arrest (p<0.05). The findings demonstrate the antiadipogenic function of selenate and the possi-ble involvement of Sepp1 and Seps1 genes in selenate-inhibited adipogenesis in 3T3-L1 cells. Keywords :
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