Abstract
We have developed a microscope with a three-dimensional auto-tracking function by simultaneous observation in two directions. The sample for the tracking was a dinoflagellate Symbiodinium. First, microscopic images were imported into a computer and processed to acquire the position of a Symbiodinium cell. Then, a motorized stage was controlled based on the position of the Symbiodinium cell. The motorized stage was moved so that the cell came to the center of the view. We developed this procedure using LabVIEW. It took approximately 500 milliseconds to process an image and to move the stage. Three dimensional tracking of a swimming Symbiodinium cell was successfully demonstrated for the cell drawing a circular trajectory. However, we could not track the cell swimming straight.
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