Abstract
We proposed a three-dimensional (3D) assembly technique and a perfusion culture system. The technique uses water transfer printing to fabricate a multilayer cellular tube for blood vessel models. A rapid construction process is necessary for building up numerous cell modules into 3D tissues that retain the tissue geometries and initial conditions of the cells. We succeeded in assembling 3D smooth muscle cells into a tube. By the perfusion culture system, this study was aimed to determine whether the tissue-engineered cellular tube maintain the normally mechanical property as the development and function. In this work, we were able to fabricate cellular tube from multilayered tissues rapidly in the suitable conditions and perfusion culture the fabricated multilayer cellular tube.
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