3P1-A08 感熱応答性ゲルプローブを用いたスフェロイドの3次元アセンブリ(バイオアセンブラ(2))

Abstract

In this paper, we achieved three dimensional (3D) assemblies of spheroids towards 3D in vitro cell structures. The 3D assembly of cells is one of the important techniques to reveal cell growth, differentiation and hystogenesis for applications in tissue engineering, drug screening, and cell characterization. The spheroids were used as building blocks for 3D structure and thermoresponsive gel was used for spheroids assembly. The mouse myoblast cells C2C12 was cultured in an ultra-low attachment surface flask and the cells were naturally aggregated to form spheroids. The thermoresponsive Gel probe (GeT probe) we proposed was employed for assembly of the prepared spheroids in 3D. The handled spheroids by GeT probe were cultured inside the thermoresponsive gel and the cell viability was confirmed. The results validate that the GeT probe can manipulate spheroids with keeping live condition of cells and achieve 3D cell assembly inside the thermoresponsive gel with arbitrary patterning.