3D-printed wound dressings containing a fosmidomycin-derivative prevent Acinetobacter baumannii biofilm formation

Abstract

Acinetobacter baumannii causes a wide range of infections, including wound infections. Multidrug-resistant A.baumannii is a major healthcare concern and the development of novel treatments against these infections is needed. Fosmidomycin is a repurposed antimalarial drug targeting the non-mevalonate pathway, and several derivatives show activity toward A.baumannii. We evaluated the antimicrobial activity of CC366, a fosmidomycin prodrug, against a collection of A.baumannii strains, using various invitro and invivo models; emphasis was placed on the evaluation of its anti-biofilm activity. We also developed a 3D-printed wound dressing containing CC366, using melt electrowriting technology. Minimal inhibitory concentrations of CC366 ranged from 1 to 64μg/mL, and CC366 showed good biofilm inhibitory and moderate biofilm eradicating activity invitro. CC366 successfully eluted from a 3D-printed dressing, the dressings prevented the formation of A.baumannnii wound biofilms invitro and reduced A.baumannii infection in an invivo mouse model.