Abstract

Plain Language SummaryIn this ultrastructural investigation of the Sprague Dawley rat pineal gland, a series of 2,731 serial sections, obtained by block-face scanning electron microscopy, have been 3-dimensional reconstructed by using the Fiji software. The data show that the neuroendocrine pinealocyte is endowed with bulbous projections with clear vesicles, separated from the cell body by a junctional complex, preventing the secretory product to target the pinealocyte from which they originate. Together with biochemical evidence for the presence of glutamate in the clear vesicles, this morphology supports the concept of a paracrine inhibitory glutamatergic system, inhibiting melatonin production in the rat pineal gland.

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