3D surface roughness characteristics for biological applications

Abstract

This paper deals with the topographic evaluation of samples made from materials applied in biomedicine. Different samples were made from Ti, TiAlV, TiNb and austenitic steel – Fe were used together with different surface morphology created. These samples were coated with a layer of TiNb alloy. The goal was to measure surface roughness in individual samples of biomaterials and to evaluate a 3D measurement of surface morphology made with a confocal microscope. Another task was to compare with appropriate software the pictures of material topography acquired at lower and higher magnification. Lower (10x) and higher (200x) magnification was used. For measured data evaluation SPIP and Gwyddion software were used. The same method of surface treatment was used but on different samples, which can lead to different results. The values obtained from topography measurements are crucial, but their formulation or evaluation can be affected by different processing methods. Our interest was to find and record the theoretically best procedure for the evaluation of measured data. Then to better understand the cell adhesion and integration of bacteria, we dealt with visualization of topography of given samples.