3D superresolution microscopy on the immunological synapse at isotropic precision below 15 nm

Abstract

T-cells are part of our adaptive immune system and are responsible for recognition of antigens in our bodies. T-cell activation is triggered upon binding of T-cell receptor (TCR) to major histocompatibility complex loaded with antigenic peptide (pMHC) which is presented on the surface of antigen presenting cells (APC). According to the kinetic segregation model of T-cell activation, T-cell topography plays a large role in the antigen recognition process. In this study we have applied a 3D superresolution method to study the spatial organization of the T-cell receptor within the immunological synapse with isotropic localization precision below 15 nm.