Abstract
Current paper presents the results of the usage of indocyanine green and pheophorbide nanoform on 2D and 3D models of FaDu cells culture. The 2D model or monolayer was used for investigation of nanoparticles distribution within individual cells and their organelles. The 3D model or multicellular tumor spheroids were used for estimation of cells’ metabolic processes by the investigation of the nanophotosensitizers fluorescence distribution within spheroid’s layers. It was shown that pheophorbide nanoparticles are accumulated in the external cell layers of spheroids, indocyanine green nanoparticles distribution demonstrates completely opposite status – in the central part of the spheroid.
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