Abstract
Optical tomography can demonstrate accurate three-dimensional (3D) imaging that recovers the 3D spatial distribution and concentration of the luminescent probes in biological tissues, compared with planar imaging. However, the tomographic approach is extremely difficult to implement due to the complexity in the reconstruction of 3D surface flux distribution from multi-view two dimensional (2D) measurements on the subject surface. To handle this problem, a novel and effective method is proposed in this paper to determine the surface flux distribution from multi-view 2D photographic images acquired by a set of non-contact detectors. The method is validated with comparison experiments involving both regular and irregular surfaces. Reconstruction of the inside probes based on the reconstructed surface flux distribution further demonstrates the potential of the proposed method in its application in optical tomography.
Highlights
As a promising molecular imaging technology, optical imaging (OI) has been attracting increased attention and been widely applied in many domains in recent years because of its significant advantages in temporal resolution, imaging contrast and sensibility, no ionizing radiation and cost-effectiveness [1,2,3]
Optical tomography has become a valuable tool for the biomedical imaging field, since it is a noninvasive imaging technique that is capable of three-dimensionally recovering the location and concentration of the luminescent probe inside a small living animal from surface measurements of the transmitted light flux [4,5,6]
We have developed a novel and practical method for 3D flux reconstruction of arbitrarily shaped surface from multi-view 2D photographic images
Summary
As a promising molecular imaging technology, optical imaging (OI) has been attracting increased attention and been widely applied in many domains in recent years because of its significant advantages in temporal resolution, imaging contrast and sensibility, no ionizing radiation and cost-effectiveness [1,2,3]. In contrast to planar imaging, optical tomography can recover the three-dimensional (3D) spatial distribution information of luminescent probes inside the animals, their location and emission powers, from measurements taken on the surface of the body. In order to measure the light flux traversing the surface, the ideal experimental setup would be such that the detectors are not in contact with the body surface Such non-contact detection system offers simple experimental procedures and enables multiview tomographic projections. An effective and practical method is lacking that reconstructs photon flux on a body surface of arbitrary shape from the measurements on a set of non-contact detectors, which is a procedure necessary in practical applications of optical tomography. We propose a novel and effective method that reconstructs photon flux on a body surface of arbitrary shape accurately from multi-view 2D photographic images measured by a CCD camera. The potential of this approach to improve the current status of optical tomography is further discussed
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