3D printing for the hla laboratory

Abstract

Aim Additive manufacturing commonly referred to as 3D printing has become accessible due to consumer grade equipment and new materials. Next generation sequencing (NGS) required the development of multiple automation accessories. These items would previously have been made using computer numerical controlled (CNC) machining. The aim of this study was to evaluate the suitability of Fused Deposition Modeling (FDM) 3D printing for design, prototyping, and fabrication of NGS automation accessories. Method An FDM 3D printer was evaluated using two types of materials (Polylactic acid (PLA), XT-Copolyester). The design, prototyping, and fabrication process was compared to CNC machining. Case 1: Adhesive foil is used to seal plates. The pierced foil binds to the tips and lifts the plate off the deck. An accessory was created to hold the plate down. Case 2: A silicon film is attached to the lid underside, preventing well to well contamination during shaking. Deck stands were developed to place the lid. Results PLA was too brittle and broke under stress. XT-Copolyester is a high strength, FDA food contact compliant material, which withstood disinfecting chemicals. 3D printing was superior to CNC machining for rapid prototyping, complex structures, entry cost, and operational safety. The 3D printer can be run unattended. CNC machining has more material choices and results in tighter tolerances. Both techniques required comparable amounts of on-instrument time. Conclusion FDM 3D printing is useful for development and rapid iterative prototyping. CNC machining is better for items requiring tight manufacturing tolerances with known material characteristics. 3D printing is a low cost alternative and produced quality parts for NGS automation. Download : Download full-size image Download : Download full-size image