Abstract
This study presents a proof of principle concept for a two-dimensional bioprinted glucose sensor on Petri dishes that allows for glucose measurements in cell culture medium. To improve bioink adhesion, the polystyrene surfaces of standard Petri dishes are activated with argon plasma, which increases roughness and hydrophilicity. The bioink containing the sensor chemistry—namely fluorescently labeled ConA/Dextran embedded in alginate microbeads—was printed on the activated Petri dishes with an extrusion-based bioprinter. The printed sensor showed good stability and adhesive properties on polystyrene. The glucose concentration was examined using a standard fluorescence microscope with filters adapted to the emission wavelength of the donor and reference dyes. The printed glucose sensor showed high sensitivity and good linearity in a physiologically relevant range of glucose concentrations.
Highlights
The field of tissue engineering aims to create two-dimensional (2D) and three-dimensional (3D) artificial cellular tissue for regenerative medicine and organ-on-chip models
This study presents a proof of principle concept for a two-dimensional bioprinted glucose sensor on Petri dishes that allows for glucose measurements in cell culture medium
A third dye can be used as a marker to provide a reference point during measurement [13,14,15]. This sensor chemistry is embedded in a hydrogel, which can be stored for many months in a dried state. Based on this optical transducer principle, we present a proof of principle concept for a bioprinted glucose sensor with good lateral resolution
Summary
The field of tissue engineering aims to create two-dimensional (2D) and three-dimensional (3D) artificial cellular tissue for regenerative medicine and organ-on-chip models. Many different types of glucose sensors have been investigated and described in literature, mostly based on optical or enzyme-based electrochemical transducer principles [2, 3]. With these sensors, glucose has been measured in blood [4, 5], in tear fluid [6, 7], in physiological fluids [5, 8] and even through skin [9, 10]. Glucose sensors for cell culture medium have been proposed [11, 12], typically based on bulky electrochemical transducers, not suitable for a sensing resolution in the millimeter range
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
