3D porous sodium alginate-silk fibroin composite bead based in vitro tumor model for screening of anti-cancer drug and induction of magneto-apoptosis

Abstract

The development of 3D scaffold-based in vitro tumor models can help to address the limitations of cell culture and animal models for designing and screening of anticancer drugs. In this study, in vitro 3D tumor models using sodium alginate (SA) and sodium alginate/silk fibroin (SA/SF) porous beads were developed. The beads were non-toxic and A549 cells had a high tendency to adhere, proliferate, and form tumor-like aggregates within SA/SF beads. The 3D tumor model based on these beads had better efficacy for anti-cancer drug screening than the 2D cell culture model. Additionally, the SA/SF porous beads loaded with superparamagnetic iron oxide nanoparticles were used to explore their magneto-apoptosis ability. The cells exposed to a high magnetic field were more likely to undergo apoptosis than those exposed to a low magnetic field. These findings suggest that the SA/SF porous beads and SPIONs loaded SA/SF porous beads-based tumor models could be useful for drug screening, tissue engineering, and mechanobiology studies.