Abstract
A 3-dimensional phase-contrast imaging technology is proposed for observation of floating cells without fluorescent labeling. In this method, a single floating cell is made to rotate and is observed by phase-contrast microscope from several directions. From these 2-D phase-contrast images, 3-D cross-sectional images are calculated by the conventional computed tomography algorithm. To rotate the cell, the proximal two-beam optical tweezers method is extended. Herein, the influence of the refractive index on light reflection and refraction occurring at the boundary surface is considered, for example, for cell organelles. The numerical aperture is optimized for the proximal two-beam optical tweezers. The method enabled successful rotation of a floating cell (breast cancer cell line) and reconstruction of 3-D phase-contrast images.
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