3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)

Abstract

Open-Top Light-Sheet (OTLS) microscopy is an emerging technique for cleared-tissue imaging that alleviates many of the physical constraints on sample size and geometry associated with conventional light-sheet microscopes. Existing OTLS designs generally use either two orthogonal objectives or a single shared objective for illumination and collection, however these architectures have various limitations for moderate NA cleared-tissue imaging. We previously developed an alternative Nonorthogonal Dual-Objective (NODO) OTLS configuration with a number of advantages for moderate-NA imaging of cleared tissues. Here we describe our latest NODO system, which is optimized specifically for high-throughput imaging of clinical samples for 3D pathology.