3D Modelling and Bioinformatics Analysis of <i>Labeo catla</i> <i>Kisspeptin1</i> and <i>Kisspeptin2</i> Proteins

Abstract

Here we characterized the Kisspeptin1 and Kisspeptin2 proteins of catla, Labeo catla ( Catla catla) - the second most important Indian major carp. The catla full-length Kisspeptin1 gene was 753 bp long which encoded a peptide of 116 AA (Amino Acids). The MW (Molecular Weight) and IP [Point Isoelectric (pl)] of catla Kisspeptin1 protein were observed to be 13371.28 kilodaltons (kDa) and 9.69, respectively. The predicted 3D structure of Kisspeptin1 protein showed a ROQF of 94.393%, and the RC Plot analysis showed that 90.35% residues were present in the most favourable region. There were 6 Serine, 2 Tyrosine and 6 Threonine P-sites, but no Ng-sites on the predicted protein were observed. Cellular localization of Kisspeptin1 protein was observed to be 31.6% in ECR, 30.8% in MR and 23 .1 % in NR. However, in the Kisspeptin2 gene, the full-length Kisspeptin2 gene was 583 bp which encoded a peptide of 125 AA. The catla Kisspeptin2 protein has an MW of 14504.60 kilodaltons (kDa) and an IP of 8.46. The predicted 3D structure of Kisspeptin2 protein showed a ROQF of 94.872% and the RC Plot analysis showed that 90.01 % residues are present in the most favoured region. There were 7 Threonine, 6 Serine, and 2 Tyrosine P-sites, but no Ng-sites were observed on the predicted protein. In contrast to Kisspeptin1 protein, 49.7% of Kisspeptin2 was observed to be present in the NR and 38.3% in the ER. The NJPT analysis showed that catla Kisspeptin1 and Kisspeptin2 amino acids showed a high similarity to Carassius auratus followed by Labeo rohita. The information generated from the present study will lay a solid infrastructure for grasp the role of Kisspeptin1 and Kisspeptin2 proteins in the NEC (Neuro-Endocrine Control) of the reproductive system in Labeo catla.