Abstract
Cervical cancer is considered the fourth most common malignant disease in women. Recently, tannin from Spatholobi Caulis (TTS) has been shown to have potent anticancer and antiproliferative characteristics in a few preliminary studies. This experiment used 3D microfluidic, flow cytometry, and gene chip technology to study the efficacy and mechanism of action of TTS, as well as molecular docking technology to study the effect of drugs on related proteins. The cell survival rates of the five groups measured by the 3D microfluidic chip were 94%, 85%, 64%, 55%, and 42%, respectively. With the increase in drug concentration, the cell survival rate gradually decreased. Apoptosis rates detected in the five groups were 2.12%, 15.87%, 33.40%, 41.13%, and 55.10%, respectively. These data suggest that TTS can promote cell apoptosis. The percentages of cells in the G0/G1 phase were 43.39%, 55.07%, 59.57%, 64.56%, and 67.39% in the five groups, respectively. TTS was demonstrated to inhibit the conversion of cells from G0/G1 to S phase and G2/M phase and inhibit gene and protein synthesis to block cell proliferation. TTS can effectively modulate pathogenic proteins. The results confirmed the efficacy of TTS against HeLa cells and that TTS can be used as an adjunct in cervical cancer prevention and treatment.
Highlights
Cervical cancer is one of the primary diseases that seriously jeopardizes the health and lives of females, according to concerning related statistics; the mortality rate of cancer has presented an increasing tendency over recent years
We looked for differentially expressed RNAs using the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases
We applied tannin extracted from Spatholobi Caulis to cells, and the experimental results show that tannin from Spatholobi Caulis (TTS) has a good therapeutic effect against cervical cancer
Summary
Cervical cancer is one of the primary diseases that seriously jeopardizes the health and lives of females, according to concerning related statistics; the mortality rate of cancer has presented an increasing tendency over recent years. Studies in the literature have shown that tannin plays important roles in many pharmacological mechanisms, such as anti-inflammatory and anticancer processes, including in breast cancer and human colorectal cancer[5,6,7]. Spatholobi Caulis is a promising anticancer drug Through this experiment, we confirmed most of the ingredients in TTS using ultra high-performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) and targeted MS/MS data acquisition strategies. Most relevant experiments have cultivated cancer cells in a two-dimensional (2D) culture system. The use of three-dimensional (3D) microfluidic in vitro models is a powerful method for screening the effects of pesticides and evaluating the mechanisms of drugs. Based on this study of its efficacy and mechanism of action, combined with modern clinical treatment, these results provide a basis for the use of TTS in the treatment of cervical cancer [Fig. 1]
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