3D Jones-maxtrix scanning of polycrystalline films of blood plasma in the diagnosis of pathological conditions of human organs.

Abstract

e15053 Background: This work is devoted to the development and experimental verification of a new method of three-dimensional matrix mapping of anisotropic properties of optically thin layers of polycrystalline films of blood plasma of biological tissues for express diagnostics and differentiation of histological sections of a prostate tumor. Methods: Biological samples. Three representative groups of polycrystalline blood plasma films were formed: 1 group consisted of blood plasma samples of polycrystalline adenoma; Group 2 consisted ofpolycrystalline adenocarcinoma, blood plasma samples are moderately differentiated (3+3 on the Gleason scale); Group 3 consisted ofpolycrystalline adenocarcinoma, blood plasma samples are poorly differentiated (4+4 on the Gleason scale). Results: The results obtained can be associated with changes in the ratio between the concentrations of albumin and globulin proteins in the blood plasma from patients with prostate tumors. It is known that malignant processes are accompanied by an increase in the concentration of optically active globulin molecules. Due to this, there is an increase in the magnitude and range of scatter in the magnitude of circular birefringence of samples of polycrystalline blood plasma films from patients with adenocarcinoma (3 + 3) and adenocarcinoma (4 + 4) in comparison with similar maps of blood plasma from patients with benign adenoma. On the contrary, due to a decrease in the concentration of albumin molecules, the values of linear birefringence of blood plasma films from patients with malignant prostate tumors decrease. Conclusions: The method of 3D Jones matrix mapping of blood plasma layers with digital holographic reconstruction of layered maps of linear and circular birefringence is analytically substantiated. The operational characteristics (sensitivity, specificity and balanced accuracy) that demonstrate the diagnostic power of 3D Jones matrix mapping of biological layers. The use of the algorithm for tomographic reproduction of linear birefringence maps provided high accuracy in differentiating benign and malignant conditions of the prostate tissue.[Table: see text]