3D images of snails at optimum energy: visualization of the embedded interior biological soft tissue, simulation and analysis

Abstract

Images of snails are obtained using synchrotron X-rays. The choice of energy is chosen based on simple approximations and with a test phantom using tube source of X-rays. The flux emitted by the X-ray tube is considerably low and the associated bremsstrahlung radiation reduces the required monochromacy. Based on the analysis, in order to improve the monochromacy and image quality, the SYRMEP facility at Elettra, Trieste, Italy and the associated detection system has been used for the image acquisition. The reconstructed image slices are assembled together to perform the volume rendering. These images are further used for analysis and extraction of parameters, for example, volume, volume fraction, occupied area of the soft tissue and the associated void space. The biological soft tissue is embedded in another object, and the microstructure is non-uniformly distributed. Under such conditions, the clear identification and occupation of the embedded biological soft tissue is necessary. The distribution of the microstructure of the biological soft tissue is clearly visible at different regions including the void space. The interior properties of the embedded soft tissue are analyzed utilizing the software to know the distribution of the attenuation coefficient in terms of density. The choice of energy region chosen on the bases of simple approximations indicate that good quality images can be obtained with less delivered doses in the field of biological imaging.