Abstract
Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1–10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 μA·mM−1·cm−2, and the detection limit (S/N = 3) was 59 μM. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.
Highlights
Diabetes is widely recognized as one of the leading causes of death and disability [1]
hydrogen titanate nanotubes (HTNTs) were directly synthesized on Ti foils by a hydrothermal treatment
Electrochemical experiments were performed with an electrochemical workstation employed the Glucose oxidase (GOx)/HTNTs/Ti electrode as the working electrode, a platinum sheet as the counter (Zahner Zennium, Germany)
Summary
Lulu Ma 1,2,† , Zhao Yue 3,† , Guona Huo 1 , Shasha Zhang 1 , Baolin Zhu 1,4, *, Shoumin Zhang 1 and Weiping Huang 1, *. College of Chemistry, National Demonstration Center for Experimental Chemistry Education (Nankai University), Tianjin 300071, China. Received: 9 January 2020; Accepted: 12 February 2020; Published: 14 February 2020
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