3D Geometric Monte Carlo Fitting of LRET Data

Abstract

We present a novel method to extract 3-dimensional conformational change within select protein systems using a Monte Carlo-based curve fitting algorithm applied to lanthanide resonance energy transfer (LRET) recordings. The key concept is to fit a constrained 3D geometry directly to a multi-exponential LRET decay. The preparation must be an n-subunit homomeric protein with each subunit containing a genetically encoded lanthanide binding tag (LBT), which holds terbium locked to the backbone of the protein.