Abstract
A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
Highlights
The fundamental property of holography is creation of 3D pictures from 2D holograms [1 - 3]
[4], it is shown both theoretically and experimentally that laser holographic microscope (LHM) is free from the speckle-noise and provides ultrahigh contrast
It should be noted that only stigmatic images were declared here and in [4] as obtained with the LHM
Summary
M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, Russia. Email address: To cite this article: Vladimir Boris Karpov. 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. Received: December 6, 2016; Accepted: August 17, 2017; Published: March 26, 2018
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
