Abstract
Experimental stacking of thylakoid membranes isolated from two plant species with different susceptibility to chilling — bean (Phaseolus vulgaris) — CS (chilling sensitive) plant and pea (Pisum sativum) CT (chilling tolerant) plant were investigated as a function of Mg2+ concentration at 0 and 6 or 8 mM MgCl2. Arrangement of CP (chlorophyll protein) complexes were analyzed using low temperature (120 K) fluorescence emission spectra of thylakoid membranes. Threedimensional structures of intact chloroplasts were obtained using confocal laser scanning fluorescence microscopy (CLSM). Experiments revealed that a reorganization of the chloroplast structure caused by changes in MgCl2 concentration is different in pea and bean.
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