Abstract
The egg yolk plasma (EYP)—a translucent fraction of the egg yolk (EY) obtained by centrifugation—was tested as a developmentally encouraging, cost-effective, biomaterial for salivary gland (SG) tissue engineering. To find optimal incubating conditions for both the human NS-SV-AC SG acinar cell line and SG fibroblasts, cells were stained with Live/Dead®. The cellular contents of 96-well plates were analyzed by high content screening image analysis. Characteristically, the EYP biomaterial had lipid and protein content resembling the EY. On its own, the EYP was non-conducive to cell survival. EYP’s pH of 6 mainly contributed to cell death. This was demonstrated by titrating EYP’s pH with different concentrations of either commercial cell culture media, NaOH, or egg white (EW). These additives improved SG mesenchymal and epithelial cell survival. The best combinations were EYP diluted with (1) 70% commercial medium, (2) 0.02 M NaOH, or (3) 50% EW. Importantly, commercial medium-free growth was obtained with EYP + NaOH or EYP + EW. Furthermore, 3D cultures were obtained as a result of EW’s gelatinous properties. Here, the isolation, characterization, and optimization of three EYP-based biomaterial combinations are shown; two were free of commercial medium or supplements and supported both SG cells’ survival.
Highlights
The acini of salivary glands (SG) are functional clusters of cells that produce saliva
The clear translucent supernatant obtained after 6 h of spinning was our desired biomaterial, the egg yolk plasma (EYP)
Our focus was to discover the requirements for human salivary glands cell survival in the translucent EYP
Summary
The acini of salivary glands (SG) are functional clusters of cells that produce saliva. Without available treatment to restore permanent salivary flow, tissue engineers want to produce implantable miniature salivary secretory units [3,4,5,6,7]. Another benefit of engineering SGs—or tissues in general—are their implementation as in vitro models that are usable in preclinical studies [8,9]. The biomaterials should provide a controlled physiologically mimicking environment and tissue growth promoting cues [10,11,12,13].
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