3D cardiac Chemical Shift Imaging of [1-13C] hyperpolarized acetate and pyruvate in pigs

Luca Menichetti,Francesca Frijia,Jan Henrik Ardenkjaer-Larsen,Vincenzo Lionetti,Rolf F Schulte,Alessandra Flori,Simone L Romano,Giulio Giovannetti,Matteo Liserani,Fabio A Recchia,Massimo Lombardi,Luigi Landini,Maria Filomena Santarelli,Giacomo Bianchi,Vincenzo Positano

doi:10.1186/1532-429x-15-s1-p10

Abstract

Background 13C Dynamic Nuclear Polarization (DNP) with rapid dissolution together with Magnetic Resonance Chemical Shift Imaging (CSI) have been used for non-invasive real-time metabolic assessment in cardiac experimental models on a clinical 3T scanner. Here, we report an in vivo comparison of hyperpolarized [1-13C] pyruvate and [1-13C] acetate perfusion and metabolism: a method based on a 3D Spiral CSI sequence is presented for obtaining spatially and spectrally-resolved information on whole heart cardiac metabolism.

Highlights

13C Dynamic Nuclear Polarization (DNP) with rapid dissolution together with Magnetic Resonance Chemical Shift Imaging (CSI) have been used for non-invasive real-time metabolic assessment in cardiac experimental models on a clinical 3T scanner
We report an in vivo comparison of hyperpolarized [1-13C] pyruvate and [1-13C] acetate perfusion and metabolism: a method based on a 3D Spiral CSI sequence is presented for obtaining spatially and spectrally-resolved information on whole heart cardiac metabolism
A graph of the g-variate and mono-exponential fitting of hyperpolarized [1-13C] acetate myocardial spectroscopic signals is reported in Figure 1 while a representative map in short axis (SA) orientation through the heart is shown in Figure 2: [1-13C] acetate is extracted inside the heart and clearly detected in the heart-chambers and myocardial wall

Summary

Introduction

13C Dynamic Nuclear Polarization (DNP) with rapid dissolution together with Magnetic Resonance Chemical Shift Imaging (CSI) have been used for non-invasive real-time metabolic assessment in cardiac experimental models on a clinical 3T scanner. We report an in vivo comparison of hyperpolarized [1-13C] pyruvate and [1-13C] acetate perfusion and metabolism: a method based on a 3D Spiral CSI sequence is presented for obtaining spatially and spectrally-resolved information on whole heart cardiac metabolism

Methods

Results

Conclusion