392 AN ASSESSMENT OF HYPOXIA IN HUMAN RENAL TUMOURS USING DIRECT OXYGEN PROBE MEASUREMENTS

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011392 AN ASSESSMENT OF HYPOXIA IN HUMAN RENAL TUMOURS USING DIRECT OXYGEN PROBE MEASUREMENTS Nathan Lawrentschuk, Aurora Poon, Lydia Johns-Putra, Carmel Murone, Ian Davis, Damien Bolton, and Andrew Scott Nathan LawrentschukNathan Lawrentschuk Heidelberg, Australia More articles by this author , Aurora PoonAurora Poon Heidelberg, Australia More articles by this author , Lydia Johns-PutraLydia Johns-Putra Heidelberg, Australia More articles by this author , Carmel MuroneCarmel Murone Heidelberg, Australia More articles by this author , Ian DavisIan Davis Heidelberg, Australia More articles by this author , Damien BoltonDamien Bolton Heidelberg, Australia More articles by this author , and Andrew ScottAndrew Scott Heidelberg, Australia More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.480AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal Cell Carcinoma (RCC) is yet to have its oxygen status formally investigated. This is despite an improved understanding of the genetic basis of RCC at a sub-cellular level (e.g. the von-Hippel Lindau axis with hypoxia-inducible factor) and the development of targeted therapies that exploit such pathways. Our aim is to measure the oxygen levels within normal kidney and RCC using direct invasive measurements. METHODS The gold standard' of oxygen tension measurement being the Polarographic Oxygen Sensor (POS) was utilized in normal and RCC tissue in patients undergoing open nephrectomy in a prospective ethically-approved study. The mean pO2Tas well as the hypoxic fraction or percentage of measurements below 5 and 10mmHg (HP5 and HP10) were recorded. Immunohistochemistry was also undertaken to analyse expression of hypoxia-related antigens. RESULTS Eleven patients successfully had POS measurements completed. Normal tissue mean pO2 was 44.95b26.06 (mmHg ±95% C.I, range 10.9–113.3) whilst tumours had a mean pO2 11.17±5.81 (0–24.9). The and also differed between Normal tissue had lower hypoxic fractions of HP10 at 21.55±12.60 (mean ±95% C.I) and HP5 16.99±11.24 compared to tumour tissue at 53.36±23.39 and 47.44±22.67 respectively (Figure 1). CONCLUSIONS Invasive POS measurements have demonstrated hypoxia in RCC consistent with that found in other solid tumours which is generally defined to occur at an oxygen tension <10mmHg (Figure 2). This in formation is imperative if we are to develop hypoxic imaging, targeted therapies and ultimately improved survival for patients with RCC. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e158-e159 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nathan Lawrentschuk Heidelberg, Australia More articles by this author Aurora Poon Heidelberg, Australia More articles by this author Lydia Johns-Putra Heidelberg, Australia More articles by this author Carmel Murone Heidelberg, Australia More articles by this author Ian Davis Heidelberg, Australia More articles by this author Damien Bolton Heidelberg, Australia More articles by this author Andrew Scott Heidelberg, Australia More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...