390 PARSTATIN ATTENUATES RENAL ISCHEMIA REPERFUSION INJURY IN RATS

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011390 PARSTATIN ATTENUATES RENAL ISCHEMIA REPERFUSION INJURY IN RATS Iason Kyriazis, Panagiotis Kallidonis, Odysseas Andrikopoulos, Nicolaos Tsopanoglou, Katerina Geronatsiou, Helen Papadaki, Despoina Liourdi, and Evangelos Liatsikos Iason KyriazisIason Kyriazis Patras, Greece More articles by this author , Panagiotis KallidonisPanagiotis Kallidonis Patras, Greece More articles by this author , Odysseas AndrikopoulosOdysseas Andrikopoulos Patras, Greece More articles by this author , Nicolaos TsopanoglouNicolaos Tsopanoglou Patras, Greece More articles by this author , Katerina GeronatsiouKaterina Geronatsiou Patras, Greece More articles by this author , Helen PapadakiHelen Papadaki Patras, Greece More articles by this author , Despoina LiourdiDespoina Liourdi Patras, Greece More articles by this author , and Evangelos LiatsikosEvangelos Liatsikos Patras, Greece More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.478AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Parstatin, is a 41 amino acid peptide that is cleaved from the proteinase-activated receptor-1 (PAR-1) during its activation by thrombin. Previous studies have demonstrated that parstatin as well as its hydrophobic N-terminal part (parstatin 1–26) demonstrate cardioprotective properties in in-vivo and in vitro experimental models of cardiovascular ischemia reperfusion injury. In this study we examine whether parstatin as well as parstatin 1–26 attenuates renal ischemia reperfusion injury (RIRI) in a rat model. METHODS In total 106 male Wistar rats were used for the purposes of this study. RIRI model included 45 minutes of bilateral renal ischemia, though clamping of both renal pedicles, followed by 4 hours of reperfusion. The effects of Parstatin on RIRI were initially examined in 77 animals divided into 8 groups including sham (vehicle/no ischemia), sham/parstatin (parstatin/no ischemia), control (vehicle pretreatment/ischemia), parstatin 3–100ìg/Kg (pretreatment with 3, 10, 30 or 100ìg/Kg parstatin/ischemia), scramble (pretreatment with a non-parstatin 41 aminoacid peptide/ischemia) and after (ischemia/administration of 30ìg/Kg parstatin after ischemia). The effects of parstatin 1–26 were then examined in 29 animals divided into 5 groups, including control (vehicle/ischemia), parstatin 1–26 1–100 ìg/Kg (pretreatment with 1, 10 or 100ìg/Kg parstatin 1–26/ischemia) and after (ischemia/administration of 10ìg/Kg parstatin 1–26 after ischemia). At the end of reperfusion period all animals were sacrificed and their kidneys, urine and blood samples were taken for histological and biochemical examination. Studied parameters were serum creatinine and BUN levels, Fractional Excretion of Sodium (FENa) and histological evaluation of renal specimens. RESULTS Administration of 10 or 30ìg/Kg of parstatin before or 30ìg/Kg after renal ischemia attenuated RIRI. Dose response study revealed that at the higher examined dose (100ìg/Kg parstatin effects were reversed. Pretreatment with 10ìg/Kg of parstatin 1–26 attenuated RIRI as well. Nevertheless, parstatin 1–26 failed to induce statistically significant nephroprotection when administered after ischemia. CONCLUSIONS Parstatin as well its hydrophobic N-terminal segment, parstatin 1–26, can preserve renal function and histological status in RIRI. The latter reveals a potential role of this molecule in clinical entities related to the phenomenon of RIRI such as partial nephrectomy. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e158 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Iason Kyriazis Patras, Greece More articles by this author Panagiotis Kallidonis Patras, Greece More articles by this author Odysseas Andrikopoulos Patras, Greece More articles by this author Nicolaos Tsopanoglou Patras, Greece More articles by this author Katerina Geronatsiou Patras, Greece More articles by this author Helen Papadaki Patras, Greece More articles by this author Despoina Liourdi Patras, Greece More articles by this author Evangelos Liatsikos Patras, Greece More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...