382 HEAT SHOCK PROTEIN 27 OVER EXPRESSION IS AN INDICATOR OF TUMOR AGGRESSIVENESS AND POOR PROGNOSIS IN RENAL CELL CARCINOMA

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011382 HEAT SHOCK PROTEIN 27 OVER EXPRESSION IS AN INDICATOR OF TUMOR AGGRESSIVENESS AND POOR PROGNOSIS IN RENAL CELL CARCINOMA Junichi Asakuma, Makoto Sumitomo, Akio Horiguchi, Takako Asano, Hidehiko Yoshii, Shinsuke Tasaki, Kenji Kuroda, Akinori Sato, Keiichi Ito, Kenji Seguchi, Masamichi Hayakawa, and Tomohiko Asano Junichi AsakumaJunichi Asakuma Tokorozawa, Japan More articles by this author , Makoto SumitomoMakoto Sumitomo Tokorozawa, Japan More articles by this author , Akio HoriguchiAkio Horiguchi Tokorozawa, Japan More articles by this author , Takako AsanoTakako Asano Tokorozawa, Japan More articles by this author , Hidehiko YoshiiHidehiko Yoshii Tokorozawa, Japan More articles by this author , Shinsuke TasakiShinsuke Tasaki Tokorozawa, Japan More articles by this author , Kenji KurodaKenji Kuroda Tokorozawa, Japan More articles by this author , Akinori SatoAkinori Sato Tokorozawa, Japan More articles by this author , Keiichi ItoKeiichi Ito Tokorozawa, Japan More articles by this author , Kenji SeguchiKenji Seguchi Tokorozawa, Japan More articles by this author , Masamichi HayakawaMasamichi Hayakawa Tokorozawa, Japan More articles by this author , and Tomohiko AsanoTomohiko Asano Tokorozawa, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.470AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The heat shock proteins 27-kDa (Hsp27) is ubiquitous chaperone molecules inducible in cells exposed to different stress conditions. Increased level of Hsp27 is reported in several human cancers, and found to be associated with poor prognosis and the resistance to some anticancer treatments. In this exploratory retrospective study, we investigated Hsp27 expression in patients with renal cell carcinoma (RCC) and its impact on clinicopathological parameters. METHODS 120 paraffin embedded RCC specimens were analyzed by immunohistochemical methods using Hsp27 monoclonal antibodies. Intensity of cytoplasmic immunostaining was scored semi-quantitatively by classifying as negative: less than 50% immunostained cancer cells, or positive: greater than 50%. The relationship between Hsp27 expression status and various clinicopathological parameters was analyzed. RESULTS Of 120 tumors 51 (42.5%) showed positive Hsp27 expression, which was significantly associated with distant metastasis, higher histological grade (G3 dominant) and regional lymph node metastasis (p<0.0001, =0.0004, 0.041), but was not with advanced pathological T stage, or microvascular invasion. Kaplan-Meier survival curves showed that patients with positive Hsp27 expression had significantly shorter cancer specific survival than those with negative Hsp27 expression (log-rank: p<0.0001). Other factors shown by univariate analysis to be significantly associated with shortened cancer specific survival were advanced pathological stage (T3-4, p <0.0001), regional lymph node metastasis (N1 or greater, p =0.0044), distant metastasis (M1, p <0.0001), higher histological grade (G3, p =0.0050) and microvascular invasion (p <0.0001). A multivariate Cox proportional hazard model analysis demonstrated that positive Hsp27 expression, and distant metastasis and microvascular invasion were independent predictors of shortened cancer specific survival (p=0.0048, 0.0065, 0.0107). CONCLUSIONS Our data suggest that increased Hsp27 expression might be an indicator of tumor aggressiveness and poor prognosis of RCC. Patients with Hsp27 positive tumors should be followed closely and carefully, and adjuvant therapy should be considered. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e154-e155 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Junichi Asakuma Tokorozawa, Japan More articles by this author Makoto Sumitomo Tokorozawa, Japan More articles by this author Akio Horiguchi Tokorozawa, Japan More articles by this author Takako Asano Tokorozawa, Japan More articles by this author Hidehiko Yoshii Tokorozawa, Japan More articles by this author Shinsuke Tasaki Tokorozawa, Japan More articles by this author Kenji Kuroda Tokorozawa, Japan More articles by this author Akinori Sato Tokorozawa, Japan More articles by this author Keiichi Ito Tokorozawa, Japan More articles by this author Kenji Seguchi Tokorozawa, Japan More articles by this author Masamichi Hayakawa Tokorozawa, Japan More articles by this author Tomohiko Asano Tokorozawa, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...