Abstract
We aimed to quantify the biological effects of an innovative Pickering emulsion coencapsulating an immunosuppressive drug (e.g.cyclosporinA) in biodegradable and biocompatible PLGA nanoparticles, with an anti-inflammatory drug (e.g.calcitriol) in an oil phase, on T-cells and keratinocytes in vitro and in a murine model of psoriasis to establish the proof of concept of emulsion efficacy in vivo. T-cells were isolated from healthy volunteer’ blood or skin explants and studied after CD3*CD28 activation during 6 days in vitro, for IL-2 production (ELISA), T cell proliferation (CFSE), and CD25 membrane expression (flow cytometry), while PMA+Ionomycin-mediated NFAT translocation was studied by immunofluorescence.
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