Abstract
This chapter describes certain approaches, which are most useful in the purification and characterization of human IL-2 and which have general applicability throughout the lymphokine field. Interleukin 2 (IL-2, T cell growth factor) is situated at the center of the cascade of antigen-nonspecific factors released during an immune response. Three methods for quantitating IL-2, a bioassay, a radioreceptor assay, and an Elisa-based assay, are described. Typical responses in each assay are illustrated. IL-2 has most frequently been quantitated by its effect upon the viability and proliferation of activated T cells. Proliferation is generally measured by [ 3 H]thymidine (Tdr) incorporation during a short pulse period. Alternatively, a combination of viability and proliferation can be measured by means of a colorimetric assay. A wide variety of target cells are used in the IL-2 bioassay. Human phytohemagglutinin (PHA) or alloantigen-activated blast cells provide one readily available source. The chapter further explains the production of IL-2.
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