Abstract
Malonaldehyde is generally measured by the 2-thiobarbituric acid (TBA) method. The TBA method, however, is not specific for free malonaldehyde, because many other substances that can occur in biological material give positive reactions with TBA. The validity of equating the TBA reaction with the content of malonaldehyde itself in the sample, however, has been questioned many times. A new high-performance liquid chromatography (HPLC) procedure for the analysis of free malonaldehyde in biological samples, such as cells and cell fractions and autoxidized fatty acids, originally developed for studying malonaldehyde formation by rat liver microsomes, is described. Even though the determination of malonaldehyde by HPLC is rather quick and very sensitive, it should not be considered as a substitute for the common TBA assay when a large number of samples must be analyzed within a short time period. In such cases, HPLC analysis of a few selected samples will generally provide sufficient information about the existence or absence of free malonaldehyde and the quantitative relationship between the TBA number and the amount of free malonaldehyde.
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