374 Eukaryotic translation initiation factor 4E (eIF4E) as a target of anti-psoriatic treatment

Abstract

Eukaryotic translation initiation factor 4E (eIF4E) has been known to play a critical role in the regulation of gene expression in eukaryotes and to affect many essential cellular processes, including proliferation, apoptosis and differentiation. We and others found eIF4E and other eIF subunits upregulated in human psoriatic skin and thus hypothesized that they are involved in the pathophysiology of psoriasis and can serve as direct topical targets for anti-psoriatic treatment. We used specific eIF4E siRNA or briciclib an eIF4E inhibitor in HaCaT cells, a human 3D-psoriasis tissue model as well as the imiquimod and TGFß mouse model to downregulate the protein and mRNA expression of eIF4E itself and its two complex partners eIF4A and G, as well as other eIFs (e.g.