Abstract
The human skin is a tightly-organized ecosystem composed of a dense network of thick structural appendices surrounding naturally-resident and recruited immune cells. Routine clinical assays, such as conventional immunohistochemistry, fail to resolve the regional heterogeneity and immune topology of inflammatory skin conditions, which can lead to false interpretation. Here we introduce MANTIS (Multiplexed Annotated Tissue Imaging System), a flexible analytical system specifically-designed for spatially-resolved immune-phenotyping of the skin in thick experimental samples or large clinical cohorts.
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