Abstract
Imaging Mass Cytometry (IMC) allows for the visualisation of up to 40 biomarkers on a single tissue section via the use of metal-tagged antibodies, permitting advanced multiplex imaging for detailed in situ analyses. To test whether this technique could be established to enhance the study of inflammatory hair diseases, we tested and optimised a panel of tissue architecture, immune infiltration, and immune activation markers on non-lesional and lesional alopecia areata (AA) frozen tissue sections.
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