Abstract
Background: HER2 is well established diagnostic and therapeutic target of the malignant tumors. It is important to precisely determine HER2 status of a tumor to achieve the best therapeutic outcome. This transmembrane receptor is shed by proteolytic cleavage and secreted as the soluble extracellular domain (ECD) of ∼97–115 kDa circulating in a patient blood. The data on HER2 protein distribution within a tumor are scarce. Therefore, our goal was to quantify the HER2 protein associated with cells and cell-free tissue fluids of tumor.
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