Abstract
Lipid hydroperoxides decompose into many secondary products including aldehydes, such as malonaldehyde (MA), which is a possible precursor for the fluorescent lipid peroxidation products formed in a variety of systems. The formation of age pigments (lipofuscin and ceroid pigments) is accompanied by the appearance of high molecular weight proteins or polymeric lipid-protein complexes, and the substances have been characterized by several investigators as having a strong fluorescence with a maximum in the region of 430–490 nm on excitation with ultraviolet light. The pigments are probably composed of heterogeneous complexes including lipid-soluble and lipid-insoluble fluorescent lipid peroxidation products. Scientific interest in fluorescent lipid peroxidation products in biological systems is rapidly developing, because it seems likely that investigation of fluorescent lipid peroxidation products leads to a clearer understanding of in vivo lipid peroxidation. This chapter introduces methods for the extraction and spectrophotofluorometric analyses of fluorescent lipid peroxidation products formed in both in vitro and in vivo systems.
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