Abstract
35Cl NMR is used to study the interaction of zinc with pyruvate kinase. The number of zinc ions bound per molecule of pyruvate kinase is determined to be 4.17 ± 0.2 and all four sites apparently have the same association constant of 0.81 ± 0.07 × 10 5 m −1. Decreases in the 35Cl-NMR line width are observed upon addition of HCO 3 −, ATP, or ADP to the zinc-pyruvate kinase binary complex. These effects could be explained by ternary complex formation or conformational changes effecting the environment of the bound zinc ion.
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