Abstract
The gene expression profile of human hematopoietic stem- progenitor cells (HSPCs) has been studied by several groups. Among the molecules differentially over-expressed in HSPCs, cell membrane proteins are of high interest, as they might allow enhanced identification and purification of HSPC subpopulations, as well as provide further insight into functional mechanisms. Based our own transcriptomic analyses of HSPCs and meta-analysis of similar studies by others (Georgantas, Cancer Research, 2004), we identified several known cell membrane genes that were over-expressed at the mRNA level in the CD34+/[CD38/Lin]lo (HSC-enriched), as compared to the CD34+/[CD38/Lin]hi (HSC-depleted, HPC-enriched), cell subset. We then used available monoclonal antibodies and multicolorflow cytometry to investigate the expression of these molecules, at the membrane protein level, on immunophenotype-defined CD34+ cell subsets from cord blood, bone marrow, and G-CSF mobilized peripheral blood.
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