330 Plant Regeneration via Organogenesis and Embryogenesis from Different Tissues of Sweetpotato

Abstract

The objective was to determine optimum conditions for embryogenic callus, embryo, organogenesis, and embryogenesis developed from leaf, petiole, stem, and tip tissues of the sweetpotato `Jewel' cultivar and from subcultured callus. Embryogenic callus was developed from stem and tip tissues on MS medium containing combinations of BA and NAA only under light conditions. Plant regeneration via organogenesis was developed from stem and tip tissues on medium including 1, 3 and 4 mg/L BA under dark and light conditions, while no plant regeneration via organogenesis was developed from leaf and petiole tissues. Frequencies for plant regeneration via organogenesis from the tissues were very low. No plant regeneration via embryogenesis was developed from the four tissues on medium having any combinations of BA+NAA and of kinetin+NAA. Embryogenic callus was observed in the subculture of callus developed from petiole and tip tissues on medium containing 0.2 and 2 mg/L 2,4-D only under dark conditions. Embryo was found in the subculture of callus from the tissues on medium containing 0.2 mg/L 2,4-D only under both conditions. Plant regeneration via embryogenesis was obtained in the subculture of callus from the tissues. Plant hormones and other factors affecting plant regeneration from the four tissues of the `Jewel' cultivar and other elite cultivars are currently being investigated at our lab for its application in transformation.